Tze-Sing Huang, Ph.D.
National Institute of Cancer Research
EDUCATIONPh. D., Biochemistry, College of Medicine, National Taiwan University, Taiwan, 1991
B. S., Plant Pathology, National Taiwan University, Taiwan, 1987
PROFESSIONAL EXPERIENCES- Assistant Investigator, Cancer Research Division, National Health Research Institutes, Taiwan
- Postdoctoral Fellow, Division of Cancer Research, National Health Research Institutes, Taiwan
- Postdoctoral Fellow, Cancer Clinical Research Center, Institute of Biomedical Sciences, Academia Sinica, Taiwan
- Visiting Postdoctoral Biologist, Department of Biology, University of California, San Diego, USA
RESEARCH INTERESTSDr. Huang's research team has recently focused on the studies of cancer proteomics and tumor-associated macrophages. They have been exploiting the strategies of proteomics to investigate the cancer protein alterations and studying their possible clinical relevance to cancer metastasis and poor prognosis. Additionally, they have learned that solid tumor is not only composed of tumor cells, rather, it comprises a range of stromal cells including fibroblasts and macrophages. They are paying much attention on the interaction between tumor cells and tumor-associated macrophages.
RESEARCH ACTIVITIES & ACCOMPLISHMENTSRecently, they have used 2D-DIGE and MALDI-TOF to identify beta-1,4- galactosyltransferase-IV differentially expressed in the tumor tissue and its adjacent non-tumor tissue of a colorectal cancer patient. By using a novel tissue lysis method and immunoblot analyses, they noted that 28% of colon cancer patients exhibited tumor beta-1,4-galactosyltransferase-IV overexpression, which was able to serve as an independent factor to predict cancer metastasis and shorter survival. On the other hand, they have successfully used an in vitro Matrigel system to prove the stimulatory effect of macrophages on the angiogenic activity of cancer cells. Interleukin-6 was first time indicated to participate in macrophage-involving tumor angiogenesis. In addition, they have found that ET-ETR axes and uPA-integrin interaction are also involved in transendothelial migration and cancer metastasis induced by tumorassociated macrophages.
- Chen WS, Chang HY, Li CP, Liu JM and Huang TS. Tumor β-1,4-galactosyltransferase-IV overexpression is closely associated with colorectal cancer metastasis and poor prognosis. Clin. Cancer Res, 11:8615-8622, 2005.
- Lin S, Huang HC, Chen LL, Lee CC and Huang TS. GL331 induces down-regulation of cyclin D1 expression via enhanced proteolysis and repressed transcription. Mol. Pharmacol, 60:768-775, 2001.
- Chao Y, Shih YL, Chiu JH, Chau GY, Lui WY, Yang WK, Lee SD and Huang TS. Overexpression of cyclin A but not Skp 2 correlates with the tumor relapse of human hepatocellular carcinoma. Cancer Res, 58:985-990, 1998.
- Kuo ML, ShenSC, Yang CH, Chuang SE, Cheng AL and Huang TS. Bcl-2 prevents topoisomerase II inhibitor GL331-induced apoptosis is mediated by down-regulation of poly(ADP-ribose)polymerase activity. Oncogene, 7:2225-2234, 1998.
- Huang, TS, Shu CH, Yang WK and Whang-Peng J. Activation of CDC 25 phosphatase and CDC 2 kinase involved in GL331-induced apoptosis. Cancer Res, 57:2974-2978, 1997.
- Huang TS, Kuo ML, Shew JY, Chou YW and Yang WK. Distinct p53-mediated G1/S checkpoint responses in two NIH3T3 subclone cells following treatment with DNA damaging agents. Oncogene, 13:625-632, 1996.
- Huang TS, Duyster J and Wang JYJ. Biological response to phorbol ester determined by alternative G1 pathways. Proc. Natl. Acad. Sci. USA, 92:4793-4797, 1995.
- Huang TS, Lee SC and Lin JK. Suppression of c-Jun/AP-1 activation by an inhibitor of tumor promotion in mouse fibroblast cells. Proc. Natl. Acad. Sci. USA, 88:5292-5296, 1991.