Faculty Profile, National Health Research Institutes, Taiwan

Faculty Profiles


Jyh-Lyh Juang, Ph.D.

Investigator
Institute of Molecular and Genomic Medicine
juang@nhri.org.tw

EDUCATION

Ph.D., Neuroscience, University of Wisconsin-Madison
M.S., Entomology, National Taiwan University
B.S., Entomology, National Chung-Hsing University

PROFESSIONAL EXPERIENCES

Associate Investigator, Division of Molecular and Genome Medicine, National Health Research Institutes, (2004-present)
Assistant Investigator, Division of Molecular and Genome Medicine, National Health Research Institutes,. (1997-2004)
Research Associate, McArdle Laboratories for Cancer Research, USA. (1994-1997)

RESEARCH INTERESTS

Dr. Juang's lab is interested in using Drosophila as a model system for understanding the conserved molecular mechanisms of human diseases. The deregulation of c-Abl kinase in cells has been implicated in the development of various human diseases. For the recent few years, they are primarily studying the involvement of Abl-Cdk5 signaling in neuronal pathogenesis of Alzheimer's disease and hand-food-and-mouth diseases. To better understand the Abl signaling, they have recently begun to study the reciprocal regulation of Abi protein localization and stability by Abl and PTP61F phosphatases in the cytoskeleton reorganization.

Another major research interest that Dr. Juang's lab has recently started is the disease diagnostic biochips. In addition to the DNA gene chip system that they have already established, they plan to expand their expertise into the frontier arenas of protein and cell chip technologies.

RESEARCH ACTIVITIES & ACCOMPLISHMENTS

Dr. Juang's research accomplishments have been fairly diverse over the years, within the broad area of neurodegeneration research, functional genomics, and recombinant baculovirus protein expression system. In neurodegeneration research: they found that Abl is involved in regulation of Cdk5 during the amyloid-initiated neurodegeneration. They first show that the suppression of Abl expression or kinase activity diminishes amyloid-triggered Cdk5 activation and cell death in neuronal cells. In the functional genomics: His lab has successfully established a comprehensive microarray facility at NHRI. By taking advantage of this technology, he has successfully developed pathogen detection gene chips for diagnosis of infectious diseases, such as SARS and HFMD (hand, foot, and mouth disease).

In recombinant baculovirus protein expression system: Drosophila S2 cells have been proven to be a useful experimental system for a high level of recombinant protein expression. His lab has developed an innovated baculovirus system for efficient protein expression in S2 cells. The USA patent of this technology has been transferred to a private sector of business. For better use of this virus in protein expression, he designed a whole-genome microarray analysis system for the investigation of the baculovirus global gene expression program to reveal a comprehensive view of the baculovirus temporal transcription program in different virus genetic backgrounds.

SELECTED PUBLICATIONS

1. Huang CH, Lin TY, Pan RL, Juang JL*. (2007) The involvement of Abl and PTP61F in the regulation of Abi protein localization and stability and lamella formation in Drosophila S2 cells. J. Biol. Chem. 282(44):32442-32452.

2. Smith I and Juang JL*. (2007) Misleading messengers? Interpreting baculovirus transcriptional array profiles. J. Virol. 81: 7819-7821.

3. Chen TC, Lai YK, Yu CK, and Juang JL*. (2007) Enterovirus 71 triggering of neuronal apoptosis through activation of Abl-Cdk5 signaling. Cell. Micorbiol. 9(11): 26762688.

4. Lin H, Lin T.Y., and Juang JL*. (2007) Abl deregulates Cdk5 kinase activity and subcellular localization for amyloid-induced Drosophila neurodegeneration. Cell Death Differ. 14:607-615.

5. Chen TC, Chen GW, Hsiung CA, Yang JY, Shih SR, Lai YK, and Juang JL*. (2006) Combining multiplex RT-PCR and a diagnostic microarray to detect and differentiate enterovirus 71 and coxsackievirus A16 infections. J Clin Microbiol. 44: 2212-2219.

6. Jiang SS, Chang IS, Huang LW, Chen PC, Wen CC, Liu SC, Lin CY, Hsiung CA and Juang JL*. (2006) Temporal Transcription Program of Recombinant Autographa Californica Multiple Polyhedrosis Virus. J. Virol. 80(18): 8989-8999.

7. Wen CC, Wu YJ, Huang YH, Chen WC, Liu SC, Jiang SS, Juang JL, Lin CY, Fang WT, Hsiung CA, and Chang IS (2006) A Bayes Regression Approach to Array-CGH Data. Stat Appl Genet Mol Biol. 5(1).

8. Chen, CW., Lin, TY., Chen, TC., and Juang JL*. (2005) Distinct translation regulation by two alternative 5 UTRs of a stress-responsive protein dPrx I. J. Biomed. Sci. 11(6):902 910.

9. Jiang, SS., Chen, TC., Yang, J. Y., Su, I J., Hsiung, CA., Liu, YL., Chen, BC., and Juang JL*. (2004) Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus (SARS-CoV) infection by real-time nested PCR. Clin. Infect. Dis. 38:293-296.

10. Juang JL*., Chen TC., Jiang SS., Hsiung CA., Chen WC., Chen GW., Lin SM., Lin JH.,Chiu S.C., and Lai YK. (2004) Coupling multiplex RT-PCR to gene chip assay for sensitive and semi-quantitative detection of severe acute respiratory syndrome-coronavirus. Lab. Invest. 84:1085-1091.

11. Lin, H., Juang JL*., and Wang, PS. (2004) Involvement of Cdk5/p25 in digoxin-triggered prostate cancer cell apoptosis. J. Biol. Chem. 279(28):29302-29307.

12. Lin T.Y., Huang C.H., Chou W.G., and Juang JL*. (2004) Abi enhances Cdc2 inhibition by Abl. J. Biomed. Sci. 11(6):902-10.

13. Lee, DF., Chen, CC., Hsu, TA., and Juang JL*. (2000) A baculovirus superinfection system: an efficient gene transfer vehicle into Drosophila S2 cells J. Virology. 74(24):11873-11880.

14. Roder, K., Hung, MS., Lee, TL., Lin, TY., Xiao, H., Isobe, KI., Juang, JL., and Shen, CK. Transcriptional Repression by Drosophila Methyl-CpG-Binding Protein (2000) Mol. Cell Biol. 20(19):7401-9.

15. Carlson, SD., Juang, JL., Hilgers, SL., and Garment, MB. (2000) Blood barriers of the insect. Ann. Rev. Ent. 45:151-174.

16. Juang JL* and Hoffmann FM (1999) Drosophila Ableson Interacting Protein (Abi) is a positive regulator of Ableson tyrosine kinase activity. Oncogene 18: 5138-5147.

(*=Corresponding Author)

PATENT

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2. Juang, J.L., Lee, D.F. "Internal ribosome entry site of the labial gene for protein expression". US Patent. Patent # US US7119187 (2006)

3. Juang, J.L., Lee, D.F. "Internal ribosome entry site of the labial gene for recombinant protein expression". ROC patent # I260346 (2006)

4. Juang, J.L., Lee, D.F. "Baculovirus-based expression system". US Patent. Patent # US6,814,963 B2. (2004)